SCREENING OF PHENOLIC CONTENT, ANTIOXIDANT AND IN VITRO EYE IRRITATION ACTIVITIES FROM APIACEAE FAMILY (DRY SEEDS) FOR POTENTIAL COSMETIC APPLICATIONSAbstract
Cosmetic applications of spices and herbs are well recognized. Apart from preservative and antimicrobial activities, spices display copious antioxidant activity. Inclusion of these antioxidants in topical supplements has proved to be effective against oxidative aging. However, food and contact allergy to most spices make it mandatory to screen for skin and eye irritation activities before cosmetic applications. In this report, we have aimed to compare ethanolic extracts of five seeds belonging to family Apiaceae (C. carvi, C. cyminum, C. sativum, T. ammi, A. graveolens) concerning their antioxidant activities, phenol and flavonoid content, in-vitro cytotoxicity, and eye irritation tests. Our investigation illustrates that C. carvi expresses the highest antioxidant activity among these. Further, the polyphenol content and antioxidant activity are positively correlated in all samples. In-vitro cytotoxic analysis on chick embryo fibroblasts and in-vitro eye irritation assays demonstrate 200µg/ml concentration of these extracts can be safely used for cosmetic application.
M. R. Shah *, K. V. Satardekar and S. S. Barve
Kelkar Education Trust’s Scientific Research Centre, Mulund (East), Mumbai, Maharashtra, India.
27 March 2014
26 May 2014
13 July 2014
01 October 2014