SCREENING OF SOY LECTIN: AS NEW ERA CANCER HEALING AGENTAbstract
The lectins are glycoproteins or sugar binding proteins of non-immune origin but are barred from sugar binding antibodies and enzymes. Lectins are isolated and purified from seeds of Glycine max by soxhlet extraction and dialysis. These collected crude lectins were centrifuged till pH is shifted downward to optimal pH for coprecipitation. Filtration of the same carried out on a Buchner funnel with a pad of Hiflo Supercel on whatman paper. Galactose was added as a ligand to the mixture kept at 250C for 10-20 min. It formed matrix coprecipitation which was centrifuged to remove additional particulates. Supernatant was removed and retained the galactose lectin coprecipitate which finally yields lectins, further purified by dialysis. Encapsulation by spray drying using maltodextrin and lactose along with the Eudragit S100 targeted the drug moiety to colon. Purified Lectins have the binding property of carbohydrate moieties on the surface of erythrocytes which agglutinate the erythrocytes, these lectins were evaluated by the agglutination test using ‘A’ positive blood group. These lectins showed anticancer activity against the colorectal type of cancer cell lines HCT- 116; proved as new link for developing anticancer drug specific to colorectal adenomas from Glycine max seeds. Calculated IC50 value by SRB cytotoxic study which was found 12 compared with capecitabine as standard anticancer drug which was 9.
A. T. Tande *, M. S. Lade , A. R. Patil , A. B. Patil and J. I. D‟souza
Tatyasaheb Kore College of Pharmacy 1, Warananagar, Kolhapur, (MS), India.
06 December, 2015
10 February, 2016
07 February, 2016
01 May 2016