SENSITIVE AND RAPID ESTIMATION OF SARECYCLINE, AN ANTIBIOTIC DRUG IN SPIKED HUMAN PLASMA BY LC-MS/MSAbstract
Sarecycline, a narrow spectrum tetracycline-derived antibiotic, is used to treat moderate to severe Acne vulgaris. The current study is aimed at developing a simple, sensitive and accurate liquid chromatography-tandem mass spectrometric (LC/MS/MS) method and validating the same for determination of sarecycline in human plasma. Zorbax, SB C18, 4.6 mm × 75 mm, 3.5 μm, 80 Å column, 10 mM ammonium formate (pH 4.5.): acetonitrile (50:50 v/v) mobile phase was used for chromatographic separation of sarecycline. The sarecycline and minocycline were monitored by electrospray ionization in positive ion multiple reaction monitoring (MRM) modes. This mode was used to detect the sarecycline and minocycline (IS) at m/z 488.6 / 291.1 and 458.4 / 337.2. Liquid-liquid extraction was employed in the extraction of analytes from human plasma. This method is validated over a linear concentration range of 0.5-150.0 ng/mL with a correlation coefficient (r) of ≥ 0.9997. Both drug and internal standards (IS) were stable in plasma samples.
B. S. Prasad * and S. J. Kumari
Department of Pharmacognosy, School of Pharmaceutical Sciences, Vel’s Institute Science, Technology & Advanced Studies (VISTAS), Pallavaram, Chennai, Tamil Nadu, India.
01 November 2019
20 December 2019
25 December 2019
01 January 2020