SESAME OIL INCORPORATED MEDIUM FOR ISOLATION AND ENUMERATION OF LIPOPHILIC YEASTSAbstract
This communication reports on an easy and cost effective mycological medium, developed for primary isolation and culture of lipophilic yeasts. The medium has been modified and adjusted specifically to promote the growth of lipophilic yeasts and simultaneously suppress the growth of other fungi and bacteria. The traditional medium of Sabouraud’s dextrose agar (SDA) with olive oil overlay was modified by incorporating different oils (coconut, palm, corn, olive and sesame) in the medium. The oil incorporated SDA medium was compared with other routinely used media such as Modified Dixon’s, IMU – Mf and Modified Leeming – Notman, for the culture of lipophilic yeasts. From the results obtained, it was observed that the medium with sesame oil exhibited the highest viable count within 48 hours. Thus, this medium can be used in the conventional microbial procedures such as isolation of pure culture by streaking; enumeration (viable count) using spread plate technique, and anti-microbial sensitivity testing, for lipophilic yeasts.
V. Agrawal*, A.M. Bhagwat and C.S. Sawant
School of Science NMIMS 1 (Deemed to be University), Mumbai, Maharashtra, India
09 January, 2014
03 March, 2014
05 April, 2014
01 July, 2014