SIMULTANEOUS DETERMINATION OF PREDNISOLONE AND ASPIRIN IN SYNTHETIC MIXTURE BY VIERORDT´s METHODAbstract
Prednisolone (10mg/day) plus low dose Aspirin (80mg/day) improve the implantation rate in women with autoimmune condition who are undergoing in-vitro fertilization. A new, simple, precise, accurate, and validated simultaneous equation method (Vierordt´s Method) has been developed for simultaneous determination of Prednisolone and Aspirin in the synthetic mixture. The method was based on the measurement of absorbance of both components at their ʎmax 243 nm and 226 nm of Prednisolone and Aspirin in methanol as solvent correspondingly. Linearity was obtained over a range of 2-6 µg/mL for Prednisone and 16-48 µg/mL for Aspirin. The percentage recovery obtained for Prednisolone and Aspirin was found to be in the range 98.85% to 99.95% and 99.41% to 99.74%, respectively. The results of the proposed method were validated for linearity, precision, accuracy, robustness, ruggedness according to ICH guideline Q2(R1). The developed method can be successfully be applied for simultaneous estimation of drugs in all commercial products.
P. Patel *, J. Patel and K. Detholia
Department of Pharmaceutical Quality Assurance, Smt. S.M. Shah Pharmacy College, Amsaran, Gujarat, India.
23 April 2020
30 August 2020
13 April 2021
01 May 2021