SIMULTANEOUS DETERMINATION OF SALBUTAMOL AND AMBROXOL IN FIXED DOSE COMBINATION BY SPECTROPHOTOMETRY
AbstractSalbutamol (SAL) and Ambroxol (AMB) is used for the treatment of asthma and bronchitis. Two simple, economical, accurate, and precise methods for simultaneous estimation of Salbutamol Sulphate (SAL) and Ambroxol Hydrochloride (AMB) in tablet dosage form have been developed. The methods are absorbance correction method (I) and first order derivative method (II). In first method ambroxol hydrochloride (AMB) was determined directly from calibration plot by measuring absorbance at 300nm and salbutamol sulphate (SAL) was determined after correction for absorbance of ambroxol hydrochloride (AMB) at 223 nm. Second method is based on first order derivative spectroscopy to overcome the spectral interference from the other drug. Wavelength 252 nm was selected for SAL and 232 nm for AMB. Both the methods showed linearity in the concentration range of 2-10µg/ml for SAL and 2-20 µg/ml for AMB. The accuracy and precision of the methods were determined and the methods validated stastically. No significant difference was observed between the results obtained by the two methods.
Article Information
19
1225-1230
568
1843
English
Ijpsr
Priyanka A. Patel, Manjusha N. Dole*, Sanjay D. Sawant, Priyanka S. Shedpure
Department of Pharmaceutical Chemistry, Smt. Kashibai Navale College of Pharmacy, Kondhwa, Pune, Maharashtra, India
31 January, 2010
18 March, 2011
06 April, 2011
http://dx.doi.org/10.13040/IJPSR.0975-8232.2(5).1225-30
01 May, 2011
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