SIMULTANEOUS ESTIMATION OF CIPROFLOXACIN AND METRONIDAZOLE IN BULK AND TABLET FORMULATION BY UV SPECTROPHOTOMETRYAbstract
Three simple and economical UV-spectrophotometric methods have been developed and validated for simultaneous estimation of ciprofloxacin (CIP) and metronidazole (MET) in a tablet dosage form using distilled water as a green solvent. The proposed methods were; simultaneous equation method (method A), Q-absorbance ratio method (method B), and area under curve method (method C). λmax of CIP & MET in distilled water were found to be 271 nm and 320 nm, respectively. The isoabsorptive point was observed at 290 nm. The linearity was obtained in the concentration range of 1-9 μg/ml, and 2-18 μg/ml for CIP and MET respectively by methods A, B & C. Validation parameters were carried out. All three methods were found to be linear, accurate, precise, and specific. Good results were achieved using distilled water as solvent due to its greater solubility, reproducible readings with maximum absorbance. Among the three methods, method C was found to be the most sensitive. Hence, this method can be recommended for the routine analysis of this drug combination.
A. Veeshma, S. Priyanka, K. P. Kumar and K. Sirisha *
Department of Pharmaceutical Analysis, Vaagdevi College of Pharmacy, Ramnagar, Hanmakonda, Warangal, Telangana, India.
03 April 2020
19 August 2020
05 September 2020
01 April 2021