SIMULTANEOUS ESTIMATION OF CURCUMIN AND ASCORBIC ACID IN POLYHERBAL FORMULATION USING UV SPECTROSCOPY
AbstractNowadays, polyherbal formulations are in demand for managing and treating many diseases owing to their less side effects. Thus, qualitative and quantitative standardization of these herbal formulations as per WHO and ICH guidelines is essential to ensure the quality and efficacy of the formulations. The present study involves the development of a simple and precise UV/Visible spectrophotometric method for simultaneous estimation of curcumin and ascorbic acid in the polyherbal antidiabetic formulation at their respective λmax 422 nm and 266 nm. The method obeys Beer’s law in the concentration range of 2 to 10 μg/mL for both curcumin and ascorbic acid with a correlation coefficient of 0.9992 and 0.999, respectively. The limits of detection (LOD) and quantitation (LOQ) were found to be 0.225 μg/mL and 0.675 μg/mL for curcumin and 0.12 μg/mL and 0.36 μg/mL for ascorbic acid, respectively. The obtained results demonstrated that the proposed method is simple, sensitive, and cost-effective and can conveniently be employed for the routine analysis of any polyherbal formulations containing curcumin (Curcuma) and ascorbic acid (Amla). The method was validated as per ICH Q2R1 guidelines.
Article Information
39
4009-4014
2289 KB
335
English
IJPSR
Shashikala Metri *, Sai Chandana Tata and Ceema Mathew
Department of Pharmacognosy, Gokaraju Rangaraju College of Pharmacy, Hyderabad, Telangana, India.
shashimetri@rediffmail.com
15 December 2022
18 March 2023
28 May 2023
10.13040/IJPSR.0975-8232.14(8).4009-14
01 August 2023
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