SIMULTANEOUS ESTIMATION OF DICLOFENAC SODIUM AND ESOMEPRAZOLE MAGNESIUM TRIHYDRATE IN BULK DRUG AND IN SYNTHETIC MIXTURE BY SPECTROPHOTOMETRY

Two simple, rapid, precise and accurate spectrophotometric methods have been developed for simultaneous estimation of Diclofenac Sodium (DIC) and Esomeprazole Magnesium Trihydrate in bulk drugs and synthetic mixture. Method A, Simultaneous equation method (Vierodt’s method) applies measurement of absorptivities at two wavelengths, 280.00 nm, (λ_max of Diclofenac Sodium) and 301.00 nm,( (λ_max of Esomeprazole Magnesium Trihydrate) in zero order spectra. The concentrations can be calculated from the derived equations. Method B, Absorbance ratio method (Q-method) involves formation of Q-absorbance equation at 302.80 nm (isoabsorptive point) and 280.00 nm (λ_max of Diclofenac Sodium) in zero order spectra. Developed methods were validated according to ICH guidelines. The calibration graph follows Beer’s law in the range of 2.0 to 10.0 μg/ml for Esomerazole Magnesium Trihydrate and 5.0 to 25.0 μg/ml for Diclofenac Sodium  with R² value greater than 0.999. Accuracy of all methods was determined by recovery studies and showed % recovery between 98 to 102%. Intraday and inter day precision was checked for both methods and mean %RSD was found to be less than 2 for both the methods. The methods were successfully applied for estimation of Diclofenac Sodium and Esomeprazole Magnesium Trihydrate in Synthetic mixture.