SIMULTANEOUS ESTIMATION OF DICLOFENAC SODIUM AND ESOMEPRAZOLE MAGNESIUM TRIHYDRATE IN BULK DRUG AND IN SYNTHETIC MIXTURE BY SPECTROPHOTOMETRYAbstract
Two simple, rapid, precise and accurate spectrophotometric methods have been developed for simultaneous estimation of Diclofenac Sodium (DIC) and Esomeprazole Magnesium Trihydrate in bulk drugs and synthetic mixture. Method A, Simultaneous equation method (Vierodt’s method) applies measurement of absorptivities at two wavelengths, 280.00 nm, (λmax of Diclofenac Sodium) and 301.00 nm,( (λmax of Esomeprazole Magnesium Trihydrate) in zero order spectra. The concentrations can be calculated from the derived equations. Method B, Absorbance ratio method (Q-method) involves formation of Q-absorbance equation at 302.80 nm (isoabsorptive point) and 280.00 nm (λmax of Diclofenac Sodium) in zero order spectra. Developed methods were validated according to ICH guidelines. The calibration graph follows Beer’s law in the range of 2.0 to 10.0 μg/ml for Esomerazole Magnesium Trihydrate and 5.0 to 25.0 μg/ml for Diclofenac Sodium with R2 value greater than 0.999. Accuracy of all methods was determined by recovery studies and showed % recovery between 98 to 102%. Intraday and inter day precision was checked for both methods and mean %RSD was found to be less than 2 for both the methods. The methods were successfully applied for estimation of Diclofenac Sodium and Esomeprazole Magnesium Trihydrate in Synthetic mixture.
Darshan Gohil and S.J. Rajput*
Quality Assurance Laboratory, Centre of Relevance and Excellence in Novel Drug Delivery System, Pharmacy Department, The Maharaja Sayajirao University of Baroda, Fatehgunj, G. H. Patel Building, Donor’s Plaza, Vadodara – 390 002, Gujarat, India
11 February, 2013
23 March, 2013
26 May, 2013
01 June, 2013