SIMULTANEOUS ESTIMATION OF DICLOFENAC SODIUM AND FAMOTIDINE BY REVERSED-PHASE THIN LAYER LIQUID CHROMATOGRAPHY / DENSITOMETRY METHOD IN BULK AND IN TABLET DOSAGE FORM
AbstractThe present work describes a validated reverse phase high performance thin layer liquid chromatographic method for simultaneous estimation of Diclofenac sodium and Famotidine in bulk and in pharmaceutical dosage form. Chromatographic separation of the drugs were performed on aluminum plates precoated with 200-μm layers of silica gel 60 RP-18 F254S as the stationary phase and the solvent system consisted of methanol: water: triethylamine (7.5:3.5:0.5) (v/v/v). Densitometric evaluation of the separated zones was performed at 291 nm. The two drugs were satisfactorily resolved with Rf values 0.54 ± 0.02 and 0.69 ± 0.02 for Diclofenac Sodium and Famotidine, respectively. The accuracy and reliability of the method was assessed by evaluation of linearity over the range of 1500 ng/band to 9000 ng/ band for Diclofenac Sodium and over the range of 500 ng/ band to 3000 ng/ band for Famotidine respectively.
Article Information
29
2677-2682
380KB
1543
English
IJPSR
Amol J. Mhaske, Jineetkumar B. Gawad* and Vijay K. Patil
Department of Pharmaceutical Chemistry, R. C. Patel Institute of Pharmaceutical Education and Research, Karwand Naka, Shirpur, Dist: Dhule- 425 405, Maharashtra, India
gawadjinit@yahoo.com
05 March, 2013
08 April, 2013
23 June, 2013
10.13040/IJPSR.0975-8232.4(7).2677-82
01 July, 2013
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