SIMULTANEOUS ESTIMATION OF ESOMEPRAZOLE AND NAPROXEN IN BULK AS WELL AS IN PHARMACEUTICAL FORMULATIONS BY USING RP-HPLCAbstract
Esomeprazole is used to treat gastro esophageal reflux disease. Naproxen is a Non-steroidal anti-inflammatory drug (NSAID) used in the treatment of pain or inflammation caused by conditions such as arthritis, ankylosing spondylitis, tendinitis, bursitis, gout, or menstrual cramps. A simple, precise, cost effective RP-HPLC method was developed and validated for the determination of both Esomeprazole and Naproxen in Pharmaceutical compositions. The chromatographic separation was achieved on a Symmetry C18 (4.6 x 150mm, 5mm, Make: XTerra) using a mobile phase consist of a mixture of phosphate buffer (pH 3) and Acetonitrile [60: 40]. The flow rate of mobile phase was maintained 1.0 mL per minute. The wavelength chosen for detection was 285 nm. The retention times of Esomeprazole and Naproxen peaks were around 2.105 and 3.555 mins respectively. The Accuracy was calculated for 50%, 100% and 150% and the % recovery was found to be 98.0%-100.4%. The method was found to be linear over the range of 5ppm to 9ppm per mL for Esomeprazole 125ppm to 225ppm per mL for Naproxen. The proposed method was validated as per the ICH and USP guidelines.
S. Ashutosh Kumar, Manidipa Debnath and J.V.L.N. Seshagiri Rao
Associate Professor, Department of Pharmaceutical Analysis, A.K.R.G College of Pharmacy, Nallajerla, West Godavari, 534112, Andhra Pradesh, India
29 March, 2013
19 June, 2013
21 July, 2013
01 August, 2013