SIMULTANEOUS ESTIMATION OF NORFLOXACIN AND TINIDAZOLE IN COMBINED TABLET DOSAGE FORM BY USING RP- HPLC METHOD
AbstractSimultaneous estimation method for Norfloxacin and Tinidazole in tablet dosage form had been developed by RP- HPLC. Both the drugs come under the category of antimicrobials and prescribed frequently in combination. The developed method is a gradient method which gives retention time of Norfloxacin and Tinidazole as 3.8min and 4.8min respectively. The separation was carried out on Supelco C18 (25cm × 4.6mm, 5µ) column using 0.05M phosphate buffer (pH 3 adjusted with ortho- phosphoric acid) and acetonitrile as a mobile phase. The flow rate was 0.8mL/min and wavelength used was 300nm. The method was validated as per ICH guideline and found to be linear between the range of 1- 6µg/mL for Norfloxacin and 1.5- 9µg/mL for Tinidazole. The developed method was found to be rapid, accurate and reproducible and therefore can be applied for simultaneous estimation of both the drugs as API or tablet dosage form effectively.
Article Information
31
236-243
535
1706
English
IJPSR
Mrunal Bulbule and Deepali Gangrade *
Vivekanand Education Society’s College of Pharmacy, Mumbai University, Chembur (E), Mumbai, Maharashtra, India
deepali.gangrade@ves.ac.in
15 July, 2016
22 August, 2016
13 September, 2016
10.13040/IJPSR.0975-8232.8(1).236-43
01 January, 2017
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