SIMULTANEOUS QUANTIFICATION OF KAEMPFEROL AND QUERCETIN IN MEDICINAL PLANTS USING HPTLC

Kaempferol and quercetin are the flavonoids which are widespread in fruits and vegetables exhibiting important biological activities. A simple and precise HPTLC method was developed and validated for simultaneous determination of kaempferol and quercetin in herbal drug extracts of Podphyllum hexandrum and Cassia angustifolia. The HPTLC of flavonoids was performed with toluene: acetone: formic acid (7:3:0.25) as mobile phases on RP-18 F254 TLC plates. Densitometric determination of flavonoids was performed at λ = 254 nm in reflectance/ absorbance mode. The results obtained indicated that the developed method was precise and accurate. Good resolution of kaempferol and quercetin was obtained from other constituents present in methanolic and hydrolysed extracts of P. hexandrum and C. angustifolia using proposed HPTLC method. Kaempferol was quantified in free (8.01and 2.42 µg/mg) as well as bound (17.54 and 8.13 µg/mg) forms and quercetin in free (4.5 and 0.93 µg/mg) as well as bound form (6.17and 4.8 µg/mg) in P. Hexandrum and C. angustifolia. Kaempferol was found to be present in high concentration and quercetin was in low concentration in these plants. Statistical data showed that method is sensitive and selective for simultaneous determination of flavonoids