SIMULTANEOUS SPECTROSCOPIC ESTIMATION AND VALIDATION OF GEMCITABINE AND TAMOXIFEN IN SYNTHETIC MIXTURE BY FIRST ORDER DERIVATIVE METHOD
AbstractA novel, simple, rapid and sensitive UV spectrophotometric method has been developed for simultaneous estimation of Gemcitabine and Tamoxifen. Gemcitabine and Tamoxifen show maximum absorbance at 269 nm and 238 nm respectively. The method employs first order derivative method. Here in this method, TAM show zero absorbance i.e Zero Crossing Point (ZCP) at 285 nm and at this wavelength GEM show measureable absorbance and ZCP of GEM is 224 nm and at this wavelength TAM show measurable absorbance. So absorbance of GEM and TAM is measured at 285 nm and 224 nm respectively. Both the drugs obey Beer’s Law in the concentration ranges employed for this method. R2 value of GEM and TAM was found to be 0.996 and 0.998 respectively. Accuracy and reproducibility of the proposed method was statistically validated by recovery studies. Limit of detection and (LOD) and limit of quantification (LOQ) of GEM was found to be 0.02833 and 0.08586 respectively. LOD and LOQ of TAM was found to be 0.09565 and 0.2898 respectively. The method is found to be rapid, precise and accurate and can easily be employed in the laboratory for the routine estimation of drugs
Article Information
41
4000-03
366
1190
English
Ijpsr
M. L. Thakkar, D. B. Shah * and D. G. Maheshwari
Department of Quality Assurance and Pharm Regulatory Affairs, L. J. Institute of Pharmacy, Ahmedabad Gujrat, India
darshilshah89@yahoo.com
10 February, 2015
19 April, 2015
30 June, 2015
10.13040/IJPSR.0975-8232.6(9).4000-03
01 September, 2015
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