SPECTRAL ANALYSIS AND HYPOGLYCEMIC EFFECT OF CRUDE SAPONIN EXTRACTED FROM PHOENIX PUSILLA ROOTAbstract
In this present study, the objective was to extract crude saponins from the root of Phoenix pusilla (PP) and to check its inhibitory potential of α- amylase and α- glucosidase enzyme. Saponins were isolated by partitioning between butanol and water. Further, the presence of saponin was confirmed by the foam test. Then the crude saponins were analyzed by UV- spectroscopy in the range of 200-400 nm and Fourier transform infrared spectroscopy (FTIR). FTIR results revealed the presence of – OH, -C-H, C=O functional groups. In-vitro antidiabetic activity assay of isolated crude saponin with acarbose as positive standard showed efficient hypoglycemic ability. Saponins have application in the food industry, cosmetics preparation and also exhibit various pharmaceutical activities. So plant-based saponin isolation in the pure form will be a boon to human utilization. This was the first report of in vitro antidiabetic potential of crude saponins of PP root.
S. Vijaya Bharathi and V. Anuradha *
Department of Biochemistry, Mohammed Sathak college of Arts & Science, Shollinganallur, Tamil Nadu, India.
02 October 2020
09 February 2021
23 June 2021
01 September 2021