SPECTROFLUORIMETRIC STUDIES ON THE INTERACTION OF MOXIFLOXACIN WITH HERRING SPERM DNAAbstract
Moxifloxacin (MOX) is a fourth-generation synthetic fluoroquinolone antibacterial agent with many important therapeutic properties. Fluorescence measurement was used to study the interaction of MOX with herring sperm DNA (Hs-DNA) in Tris-HCl, buffer of pH 7.4. The intercalative binding mode and a static quenching mechanism were confirmed by the Stern-Volmer quenching rate constant (Kq) of 5.611 x 1013 mol-1 s-1(R2 = 0.99876) at 298 K. The thermodynamic parameters (ΔH = -133.55 kJ mol–1 and ΔS = -349.98 J mol–1 K–1) were calculated at different temperatures, and they indicate that the main forces between MOX and Hs-DNA are hydrogen bonding and Van der Waals force. We proved at the same time the presence of one single binding site on Hs-DNA, and the binding constant is 1.48 x 105 M–1 at physiological pH. The results may provide a basis for further studies and clinical application of antibacterial drugs.
Babu G. Gowda*, M. Mallappa, A. Shivakumar and R. Nageshbabu
Assistant Professor, Department of Chemistry, Maharani’s Science College for Women, Palace Road, Bangalore-560 001 India.
04 May, 2014
07 August, 2014
14 August, 2014
01 December 2014