SPECTROPHOTOMETRIC DETERMINATION OF ARIPIPRAZOLE AND TAPENTADOL USING CHLORANILIC ACID REAGENTAbstract
The objective of the present work is to develop simple, precise and accurate colorimetric methods for the estimation of aripiprazole (ARP), tapentadol (TAP) using chloranilic acid reagent. ARP belongs to the class of antipsychotics. TAP is centrally acting analgesic drug. The methods are based mainly on charge transfer complexation of these drugs with p-chloranilic acid to give magenta purple colored products which were extracted into chloroform. The products were quantified at 543 nm for both ARP and TAP. The linearity of the methods was assessed in the range of 80-400 µg/ml for ARP and 200-1000µg/ml for TAP, respectively. The LOD and LOQ are 5.17 and 15.66; 82.5 and 250 for ARP and TAP, respectively. The colorimetric methods were extensively validated as per ICH guidelines and all the parameters were within the acceptance criteria with a correlation of 0.9999 and the % RSD less than 2. The results of the accuracy studies were nearer to 100%. The methods were proven to be more accurate, simple, precise and rapid by statistical validation.
Y. Naga Sri Ramya, R. Vijayalakshmi* and M. D. Dhanaraju
Research lab, GIET School of Pharmacy, Rajahmundry Andhra Pradesh, India.
20 September, 2014
19 November, 2014
11 January, 2015
01 May, 2015