SPECTROPHOTOMETRIC DETERMINATION OF DILTIAZEM IN PHARMACEUTICAL AND IN-VIVO SAMPLES WITH Pd(II)Abstract
An analytical method has been developed for quantitative determination of diltiazem, chemically (+)-cis-1,5-benzothiazepin-4-(5H)one, 3-(acetyloxy)-5-[2-(dimethyl amino)ethyl]-2,3-dihydro-2-(4-methoxyphenyl)-monohydro chloride, by complexation with Pd(II) spectrophotometrically. Diltiazem is a calcium channel blocker type antihypertensive drug. Diltiazem forms an stable orange colored 1:2 complex with Palladium(II) Chloride with lmax 400nm, molar absorptivity coefficient of complex e= 8.5 X102 l mol-1cm-1, Beer’s law range 3.413 X101 mg/ml to 2.722 X102 mg/ml with intercept of regression 0.019 and correlation coefficient 0.989. Interference of foreign metal ions and effect of temperature and pH was also studied. The characterization of complex involves, Elemental Analysis, FTIR, 1H NMR, ESR, Raman spectra, magnetic susceptibility measurement and thermal studies. On the basis of above studies structure of the complex has been proposed. The developed analytical method was applied on in-vivo blood samples- whole blood and serum samples. For whole blood samples Beer’s law range is 1.01 X 102 mg/ml to 2.77 X 102 mg/ml with coefficient of variance ±1.49 and relative standard deviation 0.64% and for serum samples Beer’s law range is 1.79X102 mg/ml to 2.47 X 102 mg/ml with coefficient of variance ±1.03 and relative standard deviation 0.79%. The procedure is rapid, accurate with precision and can be used by pathologists and in industrial sectors for determination and quality test of diltiazem in pharmaceutical samples.
Department of Chemistry, Rajdhani Engineering College, Rohini Nagar, PHASE-I, Sanaganer- Renwal Road, Jaipur, Rajasthan, India
27 July, 2013
12 September, 2013
27 November, 2013
01 December 2013