SPECTROPHOTOMETRIC DETERMINATION OF PARACETAMOL DRUG USING 8-HYDROXYQUINOLINEAbstract
A rapid and simple spectrophotometric method is reported here for the determination of paracetamol in a commercially available tablet formulation. The method is based on the diazotization of hydrolyzed paracetamol with 8-hydroxyquinoline as a coupler to form stable azo dyes color solution. The concentration of drug paracetamol was investigated by spectrophotometrically. The azo dyes formed with 8-hydroxyquinoline as coupling agents follow the Lambert-Beer’s law in the range of 2 to 10 μgmL-1 of paracetamol. Sandells sensitivity and molar absorptivity observed for azo dye coupled with 8-hydroxyquinoline was found 7.9 µg mL-1cmand 1.9 ×104 Lmol-1cm-1 respectively. The percentage recovery of the drug was found in the range of 97.4 to 100.2 % the coupling agent 8-hydroxyquinoline. The data obtained using 8-hydroxyquinoline as a coupler were compared with the data obtained with 2-naphthol as a coupler suggests that dye formed with 8-hydroxyquinoline is more stable than the 2-naphthol. The method reported here may be used to determine the trace amount of paracetamol in any clinical samples with accuracy and precision.
R.B. Dixit* and J.A. Patel
Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied Sciences, affiliated to Sardar Patel University, New Vallabh Vidyanagar – 388121, Gujarat, India
31 December, 2013
15 February, 2014
03 May, 2014
01, June 2014