SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF THIAMINE HYDROCHLORIDE IN PURE FORM, PHARMACEUTICAL AND BIOLOGICAL FLUIDSAbstract
Thiamine hydrochloride, also known as Vitamin B1, is a water-soluble vitamin which is important biologically and pharmaceutically. A new simple and sensitive spectrophotometric method has been developed for the determination of Thiamine Hydrochloride (Vitamin B1) in pure form and biological fluids. The method is based on the diazotization reaction of Thiamine Hydrochloride with Procaine HCl which reacted with sodium nitrate and hydrochloric acid to form a red water-soluble azo dye in basic medium, that has a maximum absorption at λmax = 507 nm. Beer’s law is obeyed over the concentration range (2-60 µg.ml-1), the molar absorptivity of 4.047×104 L.mol-1 .cm-1 and Sandell sensitivity of 7.417×10-3 µg .cm-2 and the method have an accuracy and precision between (0.02-0.16) while the relative standard deviation between (0.805-2.049). The structure of dye showed that (1:2) product was formed between the drug and diazotized reagent. The method was applied successfully for the determination of Vitamin B1 in pharmaceutical preparations and biological samples. The results showed that the concentration of vitamin B1were decreased, in both blood and urine samples, with time after taken a therapy.
Hind S. Al-Ward* and Saba Zuhair Hussein*
Department of Chemistry-College of Science-University of Baghdad, Baghdad, Iraq
22 May, 2016
27 June, 2016
23 August, 2016
01 October 2016