STABILITY-INDICATING SPECTROPHOTOMETRIC METHODS FOR DETERMINATION OF CEFDINIR IN PURE FORM AND PHARMACEUTICAL PREPARATIONAbstract
Three Simple, rapid, sensitive, accurate and precise stability-indicating spectrophotometric methods was developed for the determination of cefdinir in bulk powder and in pharmaceutical preparation. Method (A) Ratio derivative method(1DD); is used for the determination of intact cefdinir in presence of its degradation product at 307 nm in the range of 2 – 14 µg ml-1 with LOD of 0.049 µg ml-1 and LOQ of 0.162 µg ml-1. Method (B) Ratio difference method ;is based on measuring the difference in the amplitude of intact cefdinir in presence of its degradation product at two different wavelengths ,this is done at 219 nm and 250 nm in the range of 2 – 14 µg ml-1 with LOD of 0.050 µg ml-1 and LOQ of 0.165 µg ml-1. Method (C) First derivative method(1D); is used for the determination of intact cefdinir in presence of its degradation product at 304 nm in the range of 2 – 14 µg ml-1 with LOD of 0.034 µg ml-1 and LOQ of 0.114 µg ml-1. The methods was validated and successfully applied to the determination of cefdinir tablets with an average percent recovery ± RSD% of 100.02 ± 0.643 for method (A), 100.32 ± 0.21 for method (B) and 100.43 ± 0.86. The obtained results were statistically compared with those of the reported method by applying t-test and F-test at 95% confidence level and no significant difference was observed regarding accuracy and precision.
Khalid A. Attia, Mohammed W. Nassar, Hamed M. Abou-Seada and Mohamed Saleh Emara*
Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt
17 December, 2013
15 March, 2014
27 April, 2014
01, June 2014