STANDARDIZATION WITH HPTLC ANALYSIS OF POLYHERBAL POWDER FORMULATION: SAFOOF-E-MUSAKKIN
AbstractBackground & Objectives: Standardization of polyherbal Unani formulations is essential to assess the quality of drugs, based on their active principles, physical and chemical standards. This work reports on standardization of a polyherbal Unani powder formulation Safoofe- Musakkin (SM) consist of Rauwolfia serpentina Benth. Ex kurz root, Coriandrum sativum Linn. seed and Piper nigrum Linn. fruit. It is used in neurological disorders. Methods and Material: Plant parts were first cleaned, dried in the shade and powdered by passing through sieve # no. 80 and prepared as per the method described in UPI. SM formulation was evaluated using physicochemical tests: powder characterization, extractive value, alcohol, and water-soluble matter, Ash value, LOD at 105 ºC, pH and HPTLC fingerprinting. Statistical Analysis Used: Mean ± SEM. Result: Organoleptic characters of SM revealed brown color, characteristic odor, bitter taste, and moderately fine texture. Physicochemical parameters resulted in water-soluble extractive (9.58 ± 0.11), alcohol-soluble extractive (6.29 ± 0.07), total ash (5.87 ± 0.09), acid insoluble ash (3.09 ± 0.08), water-soluble ash (1.5 ± 0.05), LOD at 105 ºC (7.75 ± 0.058), pH of 1% and 10% solution were 6.4 ± 0.1 and 5.6 ± 0.25 respectively. The phytochemical analysis shows the presence of alkaloids, glycosides, tannins, flavonoids, steroids, terpenoids, carbohydrates, sugars, volatile oil, saponins, anthraquinones, proteins, and phenols. HPTLC fingerprinting data in two mobile phases n-Butanol: Acetic acid: water (5:1:4) and Toluene: Ethyl acetate: Formic acid (5:4:1) (v/v) was set in. Conclusion: standardization data of SM was obtained as a standard and for future reference.
Article Information
53
2478-2486
946
1035
English
IJPSR
G. J. Rather, Hamidudin *, M. Ikram, M. Zaigham, G. Sofi and T. Nayak
Department of Ilmul Saidla (Unani Pharmacy), National Institute of Unani Medicine (NIUM), Bangalore, Karnataka, India.
drhamid2003@rediffmail.com
07 September 2018
22 November 2018
24 November 2018
10.13040/IJPSR.0975-8232.10(5).2478-86
01 May 2019