STUDIES OF PRELIMINARY PHYTOCHEMICAL SCREENING, MEMBRANE STABILIZING ACTIVITY, THROMBOLYTIC ACTIVITY AND IN-VITRO ANTIOXIDANT ACTIVITY OF LEAF EXTRACT OF CITRUS HYSTRIX

In this present study, the leaf extracts of Citrus hystrix were subjected to a comparative evaluation of preliminary phytochemical screening, membrane stabilizing activity, thrombolytic activity and In-vitro antioxidant activity. The thrombolytic and membrane stabilizing activities were assessed by using human erythrocyte and the results were compared with standard streptokinase (SK) and standard anti-inflammatory drug, acetyl salicylic acid (ASA), respectively. For thrombolytic activity and membrane stabilizing activity ethanolic leaf extract exhibited clot-lysis value of 13.69% against standard SK (37.43 %) and highest percentage of hemolytic value of 74.40% against standard ASA (93.24%) respectively. DPPH free radical scavenging activity, Cupric Reducing Antioxidant Capacity (CUPRAC), Total Phenolics Content, Total Flavonoids Content, Nitric oxide scavenging assay and total antioxidant capacity were done in the present study. In case of in-vitro antioxidant evaluation all extract forms showed a significant positive results. Phytochemical investigation suggested the presence of alkaloids, carbohydrates, flavonoids, glycosides, phenols, steroids and tannins.