STUDIES ON ANTIMICROBIAL ACTIVITY OF ZIZIPHUS RUGOSA LAM. PERICARP

The desire of the present study was to investigate in-vitro antimicrobial activity on different crude extracts of Ziziphus rugosa pericarp. The antibacterial assay was carried out against Bacillus subtilis MTCC 1133, Staphylococcus aureus MTCC 7443, Escherichia coli MTCC 1692 and Salmonella typhi ATCC 19430. Meanwhile, Candida albicans ATCC 18804, Aspergillus niger ATCC 16404, Trichoderma harzianum MTCC 3832 and Polyporus rubidus MTCC 140 were used for the antifungal assay. Both activities were carried out by cup plate method using 100, 200, and 300mg/ml of hexane, ethanol, and aqueous extracts. The hexane extract showed MIC at 100 mg/ml for B. subtilis, 60 mg/ml for S. aureus and S. typhi, and 80 mg/ml for E. coli. The ethanolic extract showed 80 mg/ml for all tested organisms. The aqueous extract showed 80, 40, 60, and 100 mg/ml for B. subtilis, S. aureus, E. coli, and S. typhi, respectively. Among the extracts, aqueous extracts showed highest level activity against both gm +ve (B. subtilis 26mm) and gm -ve (S. typhi 24mm) at 300 mg/ml. Regarding antifungal activity, all the extracts at all the concentrations formed a similar zone of inhibition against A. niger. Therefore, the awesome constituents in the plant would solve some infectious as well as nutritional deficiency diseases.