STUDIES ON IN-VITRO ANTICANCER AND ANTIOXIDANT PROPERTIES FROM MARINE BACTERIAL PIGMENT ISOLATED FROM THE COASTAL AREA OF MARAKANAM (TN)Abstract
Marine bacteria have the potentiality to produce diverse bioactive molecules such as pigment. Therefore, it needs to exploit and identifying a novel type of pigment from marine bacteria for Industrial applications. This study aimed to investigate the marine bacterial pigment against antioxidant and anti-cancer properties; the marine bacteria producing pigment were isolated from water samples collected at the coastal of Marakkanam (TN), India. The isolates were screened out based on the growth characteristics and performance of different media and the strain designated as MB4, which was taken as further studies. The strain MB4 characterized by SEM analysis showed that coccoid cell morphology, nonsporulating, Gram-positive with yellow pigmentation and positive for MR-VP, catalase, lipase, acetoin production, and hemolysis. The cells were able to tolerate 10 percent NaCl concentration and ability to grown pH 9. The MB4 strain was shown a higher wave-number (1395.77) cm-1 against Raman Intensity to identify pigment production. The methanolic extracted pigment was produced at a maximum peak at 260 nm. The yellow-pigmented crude extract checked for anti-cancer properties using the colon cancer cell line (HCT15), the cell viability has been reduced after treatment of the extract (25-500 µg ml-1) and also exhibits IC50 value of 255.58 ± 43.51 mg ml-1 antioxidant DPPH radical scavenging activity. Due to their yellow pigment productions which have antioxidant activity and anti-cancer properties, this could be a novel pigment-producing strain for biomedical and industrial applications.