STUDIES ON ISOLATION, PURIFICATION AND STRUCTURE ELUCIDATION OF CHEMICAL CONSTITUENTS FROM METHANOLIC FLOWER EXTRACT OF BLEPHARIS MOLLUGINIFOLIA PERS. AND THEIR BIOLOGICAL ACTIVITIESAbstract
Blepharis molluginifolia (Acanthaceae), is a threatened medicinal herb. The methanolic flower extract of Blepharis molluginifolia was subjected to Silica Gel Column Chromatography for purification of bioactive compounds and structure elucidation of active compounds were studied. DPPH and antimicrobial activities of two isolated compounds were studied. From the analysed data, the compounds identified were 5,7-Dihydroxy-3-(4-methoxyphenyl)chromen-4-one (BiochaninA) C16H12O5 and 5,7-Dihydroxy-3-(4-hydroxyphenyl) chromen-4-one (Genistein) C15H10O5. These compounds are first time reported in this plant. Another fraction GF1 was subjected to GCMS analysis and obtained 12 compounds (aminoacid, gamma lactone, sugars and fatty acids).As GCMS profile contained majority of sugars antiglycation was done against arbutrin and found >70% inhibition. The present investigation revealed that the methanolic flower extract contained phytoestrogens like Biochanin A and Genistein, the rare aminoacid pyroglutamic acid, gamma lactones, sugars and fatty acids.
K. Deepika *, Mahesh Kumar M. V. S. , T. S. D. Radhika and S. V. Rajagopal
Department of Biotechnology, GITAM Institute of Science, GITAM University, Rushikonda, Visakhapatnam, India
23 June, 2016
26 July, 2016
02 August, 2016
01 December, 2016