STUDIES ON PHYTOCHEMICAL SCREENING – GC-MS CHARACTERIZATION, ANTIMICROBIAL AND ANTIOXIDANT ASSAY OF BLACK CUMIN SEEDS (NIGELLA SATIVA) AND SENNA ALEXANDRIA (CASSIA ANGUSTIFOLIA) SOLVENT EXTRACTSAbstract
As science encourage most of the medicinal plants as supreme source of unlimited chemical compounds which are of great interest in pharmacological preparations. The present investigation was aimed to study Nigella sativa seeds and Cassia angustifolia leaf solvent extract (petroleum ether, methanol and aqueous solutions) for phytochemical screening, GC-MS characterization, Antimicrobial and Antioxidant assay. Qualitative phytochemicals in both Nigella sativa seeds and Cassia angustifolia leaf contains high quantity (+++) of alkaloids, Carboxylic acid, Coumarins, Phenol, Resin, Saponin, and Steroid. Antimicrobial activity was determined with two gram positive (Bacillus subtilis and staphylococcus aureus) bacteria. Methanol extract of Nigella sativa seeds shown highest zone of inhibition (19.66 ± 9.29 mm) against staphylococcus aureus, Escherichia coli and klebsiella pneumonia. Petroleum ether extracts of both Nigella sativa seeds and Cassia angustifolia leaf extracts shown highest zone of inhibition against klebsiella pneumonia (20.00 ± 5.03mm and 21.66 ± 4.16 mm, respectively). From the analysis of antioxidant potential of Nigella sativa and Cassia angustifolia extracts through DPPH free radical scavenging at three different concentration (5, 10, 20 µg/ml,), Nigella sativa seed shown highest activity. The findings of the study revealed that both the traditional medicinal plants has potential phytoconstituents which triggers their biological activities, therefore these experimental plants can be further assayed for pharmacological preparations.
S. H. Reddy*, A. S. Al-Kalbani and A. S. Al-Rawahi
Applied Biology Section, Department of Applied Sciences, Higher College of Technology, Al-Khuwair, Muscat, and Sultanate of Oman.
08 May, 2017
01 January, 2018
24 January, 2018
01 February, 2018