SUBSTANTIATION OF THE PRESERVATIVE IN THE COMPOSITION OF THE IMMUNOBIOLOGICAL DRUG “CANDIDOCYDE”Abstract
Over the last decade the incidence of candidiasis has dramatically increased. To fight candidal infections vaccines with immune modulating properties have been actively investigating in recent years. In our previous studies it was found that the immunobiological drug under the conditional name “Candidocyde” based on the antigens of C. albicans and C. tropicalis fungi possessed the protective and therapeutic effect. The aim of this work is to substantiate experimentally the preservative in the composition of the immunobiological drug based on the antigens of C. albicans and C. tropicalis fungi. Various preservatives (phenol, merthiolate and formaldehyde) in the composition of the immunobiological drug have been studied. Compatibility of the preservative and the immunobiological drug was determined by the antibody titers for preventing and treating candidal infections in mice. The results of the ELISA study when preventing candidal infection have shown that after double injection of the immunobiological drug with phenol in the concentration of 0.25 % and merthiolate in the concentration of 0.01 % as preservatives there is 8 times increase the antibody titers. The immunobiological drug with formaldehyde in the concentration of 0.4 % as a preservative provided 8 times increase of the antibody titer. As a result of the research conducted it has been found that formaldehyde in the concentration of 0.4 % reduces the protective effect of the immunobiological drug, while merthiolate in the concentration of 0.01 % and phenol in the concentration of 0.25 % preserves the activity of this drug.
М. V. Rybalkin*, N. І. Filimonova, T. V. Diadiun, V. V. Kovalev and Iu. M. Azarenko
Department of Biotechnology 1, Department of Commodity 3, National University of Pharmacy, 4, Valentinovskastr., Kharkiv- 61168, Ukraine
21 September, 2015
21 January, 2016
23 January, 2016
01 March, 2016