SYNERGISM BETWEEN CASSIA FISTULA AND FLUCONAZOLE; “A NEW APPROACH AGAINST CANDIDIASIS”Abstract
Natural products have great potential against microbes and can be used to overcome the problem of drug resistant pathogens. They are helpful in reducing the toxicity of existing antifungal compounds. This present in vitro study demonstrated the synergistic effectiveness of Cassia fistula (CF) leaves extracts and fluconazole (Flu) against isolated Flu resistant strains (FRSs) of Candida albicans (CA) as the growth of FRS was found to be sensitive to extracts of CF leaves. In the present study CF leaves’ extracts were prepared in different solvents and their IC50 values were estimated by micro dilution method. It was found a statistical significant decrease in concentration of cellular lipids, phospholipid of FRS when treated against the CF leaves or combination of CF leaves and Flu. Further, H3 uptake analysis revealed that both methods affected the DNA synthesis as well as decrease in DNA contents of FRS. Hence, the results of this study would be significant for the development of new anti candidal drugs from CF leaves’ extracts in combination with known synthetic drugs like Flu to control drug resistant CA.
Yogesh Bansal*, Pratap Saini and Kirti Bansal
Department of Biochemistry, D.A.V. © Dental College and Hospital, Yamuna Nagar, Haryana, India
24 October, 2013
19 November, 2013
16 February, 2014