SYNERGISTIC ANTIBACTERIAL EFFECT OF SOME INDIAN MEDICINAL PLANTS AND COW (BOS INDICUS) URINE DISTILLATE AGAINST SELECTIVE PATHOGENIC GRAM-POSITIVE BACTERIAAbstract
Pathogenic bacterial infections are of serious clinical concern around the world. Indiscriminate use of antibiotics makes this situation beyond control. Hence, microbiologists are trying to find out the new and promising therapies against these bacterial infections. In the present research, combination therapy, which comprises extract of different medicinal plants and cow urine distillate, was used against six pathogenic gram positive bacteria. The efficacy of this combination therapy was evaluated by two methods: agar well diffusion method and XTT-colorimetric method. It has been demonstrated that CUD has profound synergistic effect on the efficacy of plants-derived extracts against the pathogenic bacteria at the specified level. The relative activity of different extracts with CUD as solvent medium was found to be increased (R.A.I= 1.61 to 2.13) against every bacteria of present study. MIC & MBC results were greatly improved (upto 2 to 3 folds) by the combination therapy. The results obtained in the present study could be useful for further research to assess the effect of combination therapy against other pathogenic microorganisms like fungi and viruses.
Vikas Pahal *, Arashdeep Kaur and Krishan Swaroop Dadhich
Department of Microbiology, Dolphin (P.G) College of Science and Agriculture, Chunni Kalan, F.G. Sahib, Punjab, India
26 May, 2016
31 July, 2016
09 August, 2016
01 November, 2016