SYNTHESIS OF SILVER NANOPARTICLES USING LEAVES AND STEM BARK OF PTEROCARPUS INDICUS AND ITS ACTIVITY ON HUMAN BREAST CANCER CELL LINE (MCF-7)Abstract
In the present study, synthesis of silver nanoparticles (AgNPs) using leaves and stem bark of Pterocarpus indicus were tested against human breast cancer cell line (MCF-7) separately. The synthesized AgNPs were characterized using UV-visible spectroscopy, X-ray powder diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), Scanning Electron Microscope (SEM) and zeta potential. The synthesized silver nanoparticles were checked with the color variation, and it was confirmed by UV-vis spectral analysis. The morphology of the synthesized nanoparticles was analyzed using SEM. The XRD was done to find out the crystalline structure of the compound. FTIR measurements are carried out to identify the possible biomolecules responsible for capping and efficient stabilization of the silver NPs synthesized using Pterocarpus indicus. The synthesized leaves-AgNPs and stem bark-AgNPs of Pterocarpus indicus were tested against the MCF-7 cell line to determine cell viability. Results showed that the stem bark-AgNPs shows more stability than leaves-AgNPs due to their higher negative zeta potential (-16.3 mV) than nanoparticles biosynthesized from leaves-AgNPs (−13.1 mV). The synthesized silver nanoparticles inhibited the proliferation of human breast cancer cell lines with an IC50 value of (126.4 μg/ml) for stem bark-AgNPs and for leaves-AgNPs (54.60 μg/ml). This study concluded that synthesized AgNPs using Pterocarpus indicus have potential anticancer activity.
Vithya Dharmaraj * and Joseph Sebastin Raj
P.G and Research Department of Biotechnology, Jamal Mohamed College (Autonomous), Affiliated to Bharathidasan University, Tiruchirappalli, Tamil Nadu, India.
15 September 2020
08 February 2021
22 June 2021
01 September 2021