THE ACTIVITY OF EURYCOMANONE DERIVATIVES ON CANCER CELL LINES

Eurycomanone from the roots of Eurycoma longifolia Jack (ELJ) has been reported to exhibit anticancer activity. Four of ester eurycomanone derivatives: eurycomanone dibutyrate, eurycomanone monovalerate, eurycomanone dimethoxybenzene, and eurycomanone disuccinate were synthesized for knowing their activities on cancer cell lines T47D, MCF-7, Hela, WIDR and Vero cells. The cancer activity was evaluated by the MTT colorimetric assay method. The results showed that eurycomanone has anticancer activity on T47D, MCF-7, Hela, WIDR cancer cells with IC₅₀ values (1.17 ± 0.09; 3.96 ± 0.02; 2.95 ± 0.08; 1.45 ± 0.01 µg/mL), and no toxic to Vero cells (609.89 ± 29.77 µg/mL). Its derivatives eurycomanone dibutyrate have anticancer activity on T47D, MCF-7, Hela, WIDR cancer cells with IC₅₀ values (25.16 ± 2.25; 21.56 ± 4.55; 29.32 ± 1.25; 149.42 ± 12.50µg/mL), eurycomanone monovalerate (25.59 ± 1.31; 22.48 ± 1.25; 30.14 ± 1.89; 91.88 ± 8.90 µg/mL), eurycomanone dimethoxybenzene ( 102.77 ± 2.56; 38.83 ± 2.55; 66.65 ± 1.90; 51.61 ± 2.37µg/mL), eurycomanone disuccinate (218.94 ± 9.30; 198.87 ± 5.50; 166.67 ± 12.34; 145.39 ± 6.67 µg/mL) respectively. The conclusion of this study showed eurycomanone dibutyrate, eurycomanone monovalerate have low activity as anticancer and safe to Vero cells. Eurycomanone dimethoxybenzoate are safe to Vero cells, besides that eurycomanone disuccinate toxic to Vero cells.