THE IN-VIVO EFFECTS OF PARTIALLY PURIFIED SUB-FRACTION (TAF2) OF THE CRUDE METHANOLIC EXTRACT OF EURYCOMA LONGIFOLIA ROOTS ON THE HAEMATOLOGICAL, BIOCHEMICAL AND HISTOLOGY PARAMETERSAbstract
Eurycoma longifolia (E. longifolia) Jack has been widely used in traditional medicine for its antimalarial, aphrodisiac, anti-diabetic, antimicrobial, anti-pyretic and anticancer activities. As yet, no study has been done on biosafety of partially purified sub-fraction (TAF2) of the crude methanolic extract of E. longifolia roots. This study aims to evaluate, in vivo, the effects of TAF2 on the haematological, biochemical and histology parameters in nude mice. Male nude mice were treated orally with TAF2 (200 mg/Kg/mouse) every other day for 16 days. Untreated mice were used as controls. Blood samples were collected by intra-cardiac puncture and haematological and biochemical parameters were analyzed by using animal blood counter and automated chemistry analyzer, respectively. Hematoxylin-eosin stain (H&E) was used to evaluate the histopathology changes. Blood cells morphology was assessed by Wright stain of the thin blood films. The results showed normal blood haematology and clinical chemistry. Histological examination of TAF2 treated vital organs (kidney, liver and spleen) of mice showed no apparent change with the dose used. In conclusion, E. longifolia root methanolicextract is safe on short-term exposur.
Omar Saeed Ali Al-Salahi , Abdel-Hamid Zaki ,Kit-Lam Chan , Amin Malik Shah , Wan Zaidah Abdullah , Narazah Mohd Yusoff*
MBBS (Malaya), DCP (London), M Med Sc (London), PhD (Kobe), Associate Professor and Hematologist, Advanced Medical and Dental Institutem, Universiti Sains Malaysia, No 6 (Lot 13), Persiaran Seksyen 4/9, Bandar Putra Bertam, 13200 Kepala Batas, Pulau Pinang, Malaysia
28 April, 2012
18 June, 2012
19 August, 2012
01 September, 2012