THROMBOLYTIC & CYTOTOXIC EFFECT OF DIFFERENT HERBAL EXTRACTSAbstract
An in-vitro thrombolytic model was used to check the clot lysis effect of four herbal extracts viz., Honey, Nigella sativa, Capsicum frutescens, Brassica oleracea, combination of Honey & Nigella sativa and Honey & Capsicum frutescens along with Streptokinase as a positive control and water as a negative control. And also brine shrimp lethality bio-assay was done using brine shrimp Nauplii and 5% of DMSO as a solvent for the ethanol extracts of Nigella sativa & Capsicum frutescens and Honey. Using an in vitro thrombolytic model, Honey, Nigella sativa, Capsicum frutescens, Brassica oleracea, combination of Honey & Nigella sativa and Honey & Capsicum frutescens showed 26.82%, 47.13%, 57.40%, 62.44%, 56.58% and 44.54% clot lysis effect respectively. From our study, we found that Brassica oleracea, Capsicum frutescens, and combination of Honey & Nigella sativa showed significant % of clot lysis effect with reference to Streptokinase. Again from in vitro brine shrimp lethality bio-assay, we found that the LC50 of Honey, Capsicum frutescens & Nigella sativa were 129.62 µg/ml, 83.33 µg/ml & 45.45 µg/ml respectively.
Md. Sheikh Anwar , Irfan Newaz Khan, Md. Mominul Islam Sarkar , Sabuj Barua , A. T. M. Mostafa Kamal , S M. Zahid Hosen
Assistant Professor, Department of Pharmacy, University of Science & Technology Chittagong (USTC), Bangladesh
09 July, 2011
11 November, 2011
19 November, 2011
01 December, 2011