TO EVALUATE THE HEPATOPROTECTIVE ACTIVITY OF DAUCUS CAROTA L AND CAMELLIA SINENSIS EXTRACT IN COMBINATION AGAINST AZATHIOPRINE-INDUCED LIVER INJURY IN RATS

Background: Daucus carota L contains a variety of carotenoids and well known for their antioxidant properties, in particular β-carotene. The antioxidant capability of polyphenols present in Camellia sinensis has been reported to be both chemo-protective and healing. The present work is about to evaluate the Hepatoprotective activity of Daucus carota L and Camellia sinensis extract combination against azathioprine-induced liver injury in rats as a model and determine the hematological findings, biochemical parameters and histophathological changes in liver section of rats. Methods: To evaluate the hepatoprotective activity of combination animals were divided into 6 groups of six rats in each group. Group I a normal control group which received normal saline on the daily basis by orally. Group II a disease group treated with drug model azathioprine 50mg/kg (w/w) i.p route to induce disease. Group III a standard drug group and the rats are treated with drug silymarin 50mg/kg (w/w) p.o Group IV test drug 1 (carrot extract) 25ml/kg body weight by p.o route. Group V test drug 2 (Green tea extract) 100mg/kg body weight by p.o route. Group VI test drugs (1+2) in combination (25ml/kg + 100mg/kg) body weight by p.o route. Results: Azathioprine showed pathological, hematological and biochemical findings in liver.  The herbal preparation prepared showed the synergistic effect and balance the level of reactive oxygen species. Conclusion: To conclude, that the Daucus carota L and camellia sinensis combination exhibits hepatoprotective activity through their antioxidant, anti-inflammatory and antiapoptotic mechanisms.