TREATMENT EFFECT OF ALCUREMON PREPARATION ON ETHANOL-INDUCED NEUROTRANSMITTERS IMBALANCE AND ALCOHOLIC LIVER DISEASE IN RATSAbstract
Alcohol use disorders (AUDs) recognized as substantial public health problem. It is grown-up many risk factors influence on alcoholism including social, behavior, an environment, and heredity conditions on the global. Alcuremon preparation was based on medicinal herbs and used for the treatment of alcohol-related disease and alcoholic liver disease in traditional Mongolian medicine. Male Wistar rats were used to induce alcoholic liver disease and chronic ethanol (EtOH) intoxication with neurotransmitter imbalance by oral administration of ethanol (subchronic 30% EtOH 10 ml/kg/day for 14 days and chronic 40% EtOH 7 ml/kg/day for 60 days). Alcuremon 50, 100 and 150 mg/kg were given orally for the same day as ethanol administration days. Some biochemical parameters and liver HE stains were examined in subchronic intoxication with alcoholic liver disease. Chronic ethanol-induced neurotransmitters changes were measured by enzyme-linked immunosorbent assay (ELISA) in brain homogenate of nucleus accumbens (NAc) and ventral tegmental area (VTA). Hippocampus was stained with cresyl violet. Data were expressed as mean ± SD. The difference between the groups was compared using one-way analysis of variance (ANOVA) followed by the Tukey’s post hoc test. Alcoholic liver damages were significantly lower in the EtOH + Alcuremon treated groups compared with the EtOH group. The EtOH + Alcuremon treated groups had a higher neuronal cell in the hippocampal CA1 and CA3 areas, and the level of the neurotransmitters was significantly balanced compared with the EtOH group. Alcuremon preparation has hepatoprotective, neuroprotective and neuromodulator effects on ethanol intoxication in rats, probably due to the presence of polyphenolic compounds.
B. E. Jargalsaikhan *, N. Ganbaatar, S. Borchuluun, A. Adilbish and C. Chimedtseren
Department of Pharmacology, Institute of Traditional Medicine and Technology, Ulaanbaatar, Mongolia.
29 December 2018
07 April 2019
19 April 2019
01 September 2019