TRYPSIN AND PROTEIN DENATURATION INHIBITORY ACTIVITY OF DIFFERENT FRACTIONATION AND ISOLATED COMPOUND OF LEAF AND ROOT OF JUSTICIA GENDARUSSA

Rheumatoid arthritis is a major ailment among autoimmune disorders. A large number of herbal extracts were used for the treatment of various types of rheumatoid disorder. The leaf and root of Justicia gendarussa belonging to Acanthaceae family, commonly known as Nilinirgundi is traditionally used for chronic rheumatism. The present study deals with in-vitro anti-arthritic activity in pharmacological models were studied such as inhibition of protein denaturation and trypsin (proteinase) inhibitory activity. Different fractions and isolated compound from methanolic extract of leaf and root of J.gendarussa with different concentrations (10,100,1000µg/ml ) were studied and results were compared with standard drug Indomethacin. The methanolic extract of leaf and root of J. gendarussa showed dose dependent activity which was found comparable to that of standard drug Indomethacin. Isolated compound-La of leaf of J. gendarussa inhibited the activity of trypsin with IC₅₀ values 34.85 µg/ml as compared to standard drug Indomethacin with IC₅₀ value 11.57 µg/ml. Compound-La of leaf of J. gendarussa showed protein denaturation inhibition at the IC₅₀ value of 24.74 µg/ml as compared to the standard drug Indomethacin with IC₅₀ value 19.41 µg/ml.