TWO NEW METABOLITES; 7β-OH-UZARIGENIN AND 7β-OH-ODOROSIDE-H; THROUGH BIOTRANSFORMATION OF UZARIGENIN AND ODOROSIDE-HAbstract
Two new cardenolide metabolites, 7β-OH-uzarigenin (1M) and 7β-OH-odoroside-H (2M) were obtained through the biotransformation of uzarigenin (1) and odoroside-H (2). Compounds 1 and 2 were isolated from the air-dried roots of Nerium oleander L. The plant, belonging to family Apocynaceae, was collected in October, 2010 from Mansoura university garden in Egypt. The methylene chloride extract of the N. Oleander roots was chromatographed using silica gel column, gradually eluted by petroleum ether: Ethyl acetate with increasing proportions of EtOAc. Further purification by repeated crystallization from hot methanol afforded both compounds. The biological transformation was acheived by Cunninghamella echinulata NRRL 1382 with different incubation periods. The structures of the isolated compounds and the newly assigned metabolites were identified using extensive studies; (1H, 13C, HSQC, HMQC, 1H-1H COSY and 1H-13C HMBC), mass spectral analysis (EI and ESI /MS) and IR.
Ahmed A. Zaki *, Nadia E. Hashish , Mohamed A. Amer and M.-F. Lahloub
Pharmacognosy Department, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt
23 April, 2014
14 June, 2014
27 July, 2014
01 November, 2014