USING AN IMMUNOINFORMATIC APPROACH, IDENTIFICATION OF B-CELL ENVELOPE PROTEOME FOR MULTIPATHOGENIC DENGUE AND ZIKA VIRUSESAbstract
Mosquitoes are carriers of the DENV and ZIKV diseases. DENV and ZIKV are epitopes. The current performance and technique of some of the most frequently used B-cell epitope predictors used for DV and ZV proteomes are reviewed in this paper. Our specific prediction methods, ABC pred, BC pred, and AAP Methods, as well as FBC pred, were utilized to predict 1529 B cell epitopes. For the B cell epitops, the stability index, aliphatic index, GRAVY, antigen city, allergen city, toxicity, clustering, and conservancy were investigated further. The anticipated immunogenic epitopes PLPWHAGADTGTPHWNNKEA, GGFGSLGLDCEPRTGLDFSD of ZIKV and IGVEPGQLKLSWFKKGSSIG, VEPGQLKLSWFKKGSSIGQM, IEAKLTNTTTASRCPTQGEP, LPLPWLPGADTQGSNW of DENV were 100 percent conserved. The goal of this study is Flaviviridae viruses that now affect 128 nations throughout the world. And they may both be fatal and life-threatening. A quicker and less expensive vaccine design procedure used to identify the B cell was to use computer simulations to find potential vaccination candidates and antiviral efficacy testing. The study’s goal is to develop patient therapies that are both safe and effective.
Neeraj Kumar Dixit
Department of Biotechnology, Saroj Institute of Technology & Management, Lucknow, Uttar Pradesh, India.
14 December 2021
19 January 2022
28 April 2022
01 August 2022