VALIDATING THE PROPERTIES OF NYMPHAEA PUBESCENS WILLD. BY PERFORMING UV-VIS, TLC, FTIR AND HPLC ANALYSESAbstract
Introduction: Nymphaea pubescens Willd. is one of the well-known medicinal plants, being used from ancient times in India and commonly called “Kumuda”. It is an aquatic perennial herb. It has leaves having a smooth upper side, a strong root, and a branched rhizome. According to Bentham and Hooker classification “Genera Plantarum”, this plant belongs to the division Polypetale of Dicotyledones, which has been divided into 15 order and 84 families. Nymphaea comes under the Araceae family, which itself contains 8 genera and 100 species. Method: Morphological characters have been examined through a transverse section cut by microtome. Physiochemical studies and other screenings were performed accordingly. In addition to this, UV-VIS, TLC, FTIR, and HPLC tests were also carried out for further validation. Result: The morphological and physiochemical examination revealed the presence of saponins, proteins, and various other tests in Nymphaea pubescens Willd. Conclusion: The microscopic, UV-VIS, TLC, FTIR, and HPLC validation can be helpful for using it for medicinal and commercial purposes.
Madhulika *, S. Kumar, K. N. Dwivedi and S. Gehlot
Department of Kriya Sharir, Faculty of Ayurveda, Institute of Medical Science, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
20 January 2020
14 April 2020
22 April 2020
01 January 2021