VALIDATION OF ABACAVIR SULFATE IN PHARMACEUTICAL DOSAGE BY REVERSE PHASE HPLC WITH INTERNAL STANDARD METHODAbstract
A rapid, specific and accurate isocratic HPLC method was developed and validated for the assay of abacavir sulfate in pharmaceutical dosage forms. The assay involved an isocratic – elution of abacavir sulfate in Grace C18 column using mobile phase composition consists of (38:62 v/v) of methanol and 10ml of potassium dihydrogen orthophosphate. The wavelength of detection is 255nm.The method showed good linearity in the range of 10-50.0mg/mL. The runtime of the method is 8 mins. The proposed method can be used for routine quality control samples in industry in bulk and in finished dosage forms. In present study, a rapid specific precise and validated HPLC method for the quantitative estimation of abacavir sulfate in pharmaceutical dosage forms has been reported. The developed method can be applied to directly and easily to the analysis of the pharmaceutical tablet preparations. The percentage recoveries were near 100% for given methods. The method was completely validated and proven to be rugged. The excipients did not interfere in the analysis. The results showed that this method can be used for rapid determination of abacavir sulfate in pharmaceutical tablet with precision, accuracy and specificity.
B. Venkata Kiran, Battula Sreenivasa Rao* and Som Shankar Dubey
Department of Chemistry, GITAM Institute of Technology, GITAM University, Visakhapatnam- 530 045, Andhra Pradesh, India
03 April, 2012
10 May, 2012
19 July, 2012
01 August, 2012