VALIDATION OF NOVEL AND COST EFFECTIVE SPECTROSCOPIC METHODS FOR ESTIMATION OF KETOROLACAbstract
Sophisticated analytical methods like HPLC and Mass spectroscopic methods which being employed for analysis, are relatively expensive and hence there is a need for developing simple analytical methods. In the proposed work, such methods have been developed and applied for routine determination of ketorolac in pharmaceutical formulations and bulk dosage forms. These methods were based on the formation of colored species on binding of ferric chloride and Hydrochloric acid for method A and binding of drug and Folin Cioclataeu reagent in alkaline conditions for method B, and the colored chromogen obtained in each method was finally treated with the drug ketorolac to produce yellow colored for method A and blue colored chromogen for method B with λmax at 430 nm and 627 nm respectively. Statistical analysis of these methods exhibited Sandell’s Sensitivity of 0.8 and 0.0617 (Method A and B) respectively, and the relative standard deviation (RSD) of these methods were found equal to 0.69 and 1.90 respectively, indicating that these methods are highly reproducible, based on the principle of absorption visible spectrophotometry for the determination of ketorolac in formulations and bulk dosage forms.
Vijaya Saradhi Settaluri *, Sheikha Saleh Abdullah Al-Salmani and Noora Hamed Nasser Al-Baddaei
Department of Applied Sciences, Higher College of technology, Al-Khuwair, PO Box: 74, Postal Code: 133, Muscat, Sultanate of Oman.
12 May, 2015
30 July, 2015
28 August, 2015
01 November, 2015