VARIOUS MEDICINAL PLANTS: A PROMISING TREATMENT FOR MULTIDRUG-RESISTANT BACTERIA ISOLATED FROM WOUND INFECTIONAbstract
Bacteria have proved remarkably developing resistant to a known antibiotic; however, Medicinal plants have manifested notably useful for the control of bacterial infections. This study aimed to isolate and identify the multidrug-resistant bacteria from wound infection, then examine the antibacterial activity of different concentration of the ethanolic extracts of Cymbopogon proximus, Azadirachta indica, and Grewia senegalensis plants against it. One hundred specimens collected from wound infection, isolation and identification done according to standard methods. Antibiotics susceptibility test performed using the Kirby-Bauer disc diffusion method for: Cotrimoxazole (25 μg) Ciprofloxacin (5 μg), Gentamicin (10 μg), Imipenem (10 μg), Erythromycin (15 μg), Tetracycline (30 μg) Methacillin (5 μg), Ceftazidime (30 μg). 94 isolates showed bacterial growth, 40 (42.6%) isolates were Gram-positive bacteria (Staphylococcus aureus) and 54 sample Gram-negative bacteria; 15 (27.8%) Pseudomonas aeruginosa, 13 (24.1%) Proteus spp, 12 (22.2%) Escherichia coli, 10 (18.5%) Klebsiella pneumonia, 4 (7.4%) Citrobacter freundii. Ethanolic extracts from Sudanese medicinal plants namely Cymbopogon proximus, Azadirachta indica, and Grewia senegalensis, screened for their antimicrobial properties against isolated multidrug-resistant bacteria at different concentrations; 100, 50, 25, 12.5 and 6.25 mg/ml using agar disc diffusion and agar well diffusion method. The result revealed that all plants extracts were active against most of the resistant bacteria with MIC ranges from 50-6.25 mg/ml. In conclusion, the number of multidrug-resistant bacteria in wound infection was high, and the tested plant’s species have a promising level of activity against multidrug-resistant bacteria. Therefore, manufacture these extracts at pharmacological form such as topical ointment should also be considered.
E. H. O. Moglad *, S. K. A. Boon and H. T. O. Ali
Department of Pharmaceutics, College of Pharmacy, Prince Sattam bin Abdulaziz University, Alkharj, Saudi Arabia.
05 May 2019
24 August 2019
01 September 2019
01 February 2020