ZINC OXIDE NANOPARTICLES INDUCED HAEMOLYTIC CYTOTOXICITY IN HORSE RED BLOOD CELLSAbstract
Metal oxide in the nanorange acquires distinctive properties that depend on size, chemical composition and surface chemistry. Among the metal oxide nanoparticles, the zinc oxide nanoparticles (ZnO NPs) are commonly used for their antimicrobial properties. ZnO nanomaterials are incorporated into a variety of skin coatings because of their antimicrobial and/or antifungal properties. Most of the future therapeutic applications of NPs are based on intravenous/oral administration. Experiments on their interaction with blood components especially erythrocytes are of immense importance, if the nanoparticles are to be administered intravenously. Haemolytic potential of ZnO NPs was assessed spectrophotometerically as well as by phase contrast microscopy. In our study, interaction of different concentrations of ZnO NPs with erythrocytes revealed absence of hemolysis by spectrophotometric method. On the other hand, phase contrast microscopic examination revealed concentration dependent clustering of erythrocytes. The detailed investigation about interaction of erythrocytes with ZnO NPs is needed before their clinical applications.
R Raguvaran, Anju Manuja*, Sandeep Singh, Meenu Chopra , Balvinder K. Manuja and U. Dimri
National Research Centre on Equines, Sirsa road, Hisar, Haryana, India
17 June, 2014
19 January, 2015
12 February, 2015
01 March, 2015