Posted by admin on Mar 31, 2020 in |
Globally, the outbreak of antibiotic-resistant pathogens derived infections urged an intensifying necessity for the survey of underexplored marine zone microorganisms for novel metabolites. Our current study emphasized on isolation, polyphasic characterization and bioactivity of the novel actinomycetes from the Bay of Bengal near Kakinada and Nizampatnam, Andhra Pradesh, India, which could provide lead bioactive compounds of therapeutic efficacy to repress the of pervasiveness multidrug-resistant pathogens. A total of fifteen actinobacterial strains (VJRM-1 to VJRM-15) were isolated and tested. Micro-morphological characteristics of the isolates were perceived by SEM (scanning electron microscope) analysis. Phylogenetic analyses of isolates were appraised with 16S rRNA gene sequence homology. The dendrogram was contrived by deploying the parsimony method in MEGA software version 6. Based on polyphasic studies, strains VJRM-7 and VJRM-8 identified as Nocardiopsis dassonvillei (KP299158) and Nocardiopsis sp (KP299159). In-vitro antimicrobial activity of crude ethyl acetate extracts derived from these strains revealed strong antagonistic activity against Gram-positive, Gram-negative bacteria and fungi. Both strains displayed positive results for the production of enzymatic activities. The morpho-physio-molecular...
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Posted by admin on Mar 31, 2020 in |
Objective: Present research work describes an eco-friendly and quick method for the development of silver nanoparticles (AgNP) using Momordica charantia Linn. extract and silver nitrate solution. Our investigation reports the antimicrobial activity of developed nanoparticles against Staphylococcus aureus, Escherichia coli. Method: Silver nanoparticle developed by reduction of silver nitrate solution by Momordica charantia Linn. extract. Extract of Momordica charantia Linn. acts as a reducing agent. Ulta Violet Visible spectroscopy used as a confirmatory test for the development of silver nanoparticles. Result: The silver nanoparticles synthesized were confirmed by their change of color to dark brown due to the phenomenon of surface plasmon resonance. The characterization studied was done by UV-visible spectroscopy, Scanning electron microscopy (SEM), X-Ray diffraction (XRD), Fourier Transmission infrared spectroscopy (FTIR), and study antibacterial activity. FTIR gives information about functional groups present in the synthesized silver nanoparticles. X-ray diffraction studies indicate that developed silver nanoparticles are highly crystalline in nature. SEM of synthesized AgNP shows that particles are spherical in shape and uniformly distributed. Synthesized AgNP shows...
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Posted by admin on Feb 29, 2020 in |
Indian literature relating to the spectrum of infections in maintenance hemodialysis patients has not been extensively published. The purpose of this study was to carry out surveillance of infections in this population and to determine the clinical patterns and outcomes of infections and also to assess the prescribed antibiotic therapy. This was a prospective observational study carried out in the dialysis unit of the nephrology department of a tertiary care teaching hospital. The sample of the study comprised of 30 patients who were receiving maintenance hemodialysis and presented with infections. Escherichia coli was the commonly detected gram-negative pathogen in culture and sensitivity tests. Whereas the most commonly isolated gram-positive bacteria were streptococcus pneumonia and methicillin-resistant staphylococcus species which were sensitive to vancomycin. Respiratory system (47.2%) followed by the urinary system (27.3%) were most commonly affected. The most common outcome of infections was a cure (57%). Clarithro-mycin and ofloxacin-ornidazole were found to be inappropriately dose adjusted. Amoxicillin-clavulanate, cefixime, ticarcillin-clavulanic acid, levofloxacin, piperacillin-tazobactam, mero-penem and imipenem-cilastatin were inappropriately adjusted for dosing...
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Posted by admin on Feb 29, 2020 in |
Dairy and milk consumption are habitually included as important essentials in a healthy and balanced diet. It is the principal food for mammals and provides all the essential energy and nutrients to guarantee proper growth and development. Whey protein present in the milk is quickly digested and therefore amino acids are delivered quickly to muscles to help start the muscle-building process. The major components of milk whey of nutraceutical potential include beta-lactoglobulin, alpha-lactalbumin, bovine serum albumin (BSA), lactoferrin (Lf), immunoglobulin’s (Igs), lactoperoxidase (Lp) enzymes, glycomacropeptides (GMP), lactose and minerals. The main aim of this study is to determine the antimicrobial and antioxidant activity of whey protein concentration from cow’s milk. The result of the present study reveals the fact that the whey milk exhibited good antimicrobial activity. Whey protein α – lacto albumin and β – globulin were confirmed by two peaks with the retention time of 3.96 and 9.45. Thus, HPLC confirmed the presence of protein in the whey milk...
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Posted by admin on Feb 29, 2020 in |
Periodontitis is a group of inflammatory pathological conditions involving periodontal ligaments and supporting alveolar bone which may result in the degeneration of supporting structures of teeth ultimately leading to tooth loss. Present research work is focused on the formulation, optimization and evaluation of chitosan microspheres and chitosan- hydroxyapatite biocomposite microspheres to have controlled release of herbal drug quercetin, of which anti-microbial, anti-inflammatory and wound healing properties are well proven. Mucoadhesive chitosan would retain the drug-loaded microspheres at the affected site and hydroxyapatite used in its combination would improve mechanical strength, osteoconductivity and sustain the release of the drug. Microspheres were formulated by solvent-free precipitation technique using the alkaline solution (NaOH solution). These microspheres were optimized using 32 factorial design using design expert software. Optimized chitosan microspheres (QC5) and chitosan-hydroxyapatite microspheres (QCH5) were characterized for surface morphology by SEM and were found to be spherical with a particle size of 5.56 µm and 7.22 µm and entrapment efficiency of 80.74 and 82.97% respectively. The entrapment of drug in microspheres was...
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