Posted by admin on Jul 31, 2020 in |
The application of nanobiotechnology is an emerging area of nanoscience and nanotechnology. In the present studies, tungsten nanoparticles were synthesized and reduced chemically, and character-rization was done by UV, SEM, TEM, FT-IR, and XRD. The size of nanoparticles was found to be 20 nm. Further, these nanoparticles were teste for various biological applications. In antimicrobial activity, it was observed that potent efficacy was observed against Streptomyces grisveus (12 mm) at 80 µg/ml while in fungus maximum activity was observed against Penicillium funicolusum (24 mm) at the same dose. The antiplatelet activity of nanoparticles was investigated by Prothrombin (PT) and Activated Partial Thromboplastin Time (APTT). In both PT and APTT assay, maximum activity was observed at 40 µg/ml (295 sec and 80 sec, respectively). Cytotoxicity was also studied by MTT assay against various cell lines. Against MCF-7, potent activity was observed at 200 µg/ml, while in 3T3 it was observed at 500 µg/ml. Finally, it was observed that these nanoparticles have potent activity against tuberculosis at 1000 µg/ml. The result...
Read More
Posted by admin on Jul 31, 2020 in |
Antimicrobial drug resistance is one of the major threats due to the wide usage of broad-spectrum antimicrobial drugs in the general population. The present study was undertaken to investigate the antibacterial potential of Alternanthera philoxeroides methanolic extract (APME) against multidrug-resistant UTI pathogens. The preliminary phytochemical screening of the extract showed the presence of carbohydrates, amino acids, proteins, alkaloids, glycosides, steroids, flavonoids, tannins, and phenolic compounds. The quantitative estimation of total phenolic, tannin, and flavonoid contents was determined for the crude extract of APME through standard curves of gallic acid and rutin. The phenolic content of APME was found to be 12.4 mg of gallic acid equivalent (GAE)/g of extract. The tannin content was found to be 5.6 mg of GAE/g of extract, whereas the flavonoid content was found to be 3.2 mg of rutin/g of extract. The antibacterial potential of the extract was observed as maximum against Staphylococcus saprophyticus, moderate against Escherichia coli and Proteus vulgaris, and minimum against Proteus mirabilis. The minimum inhibitory concentration was ranged from 12.5...
Read More
Posted by admin on Jun 30, 2020 in |
Objectives: The intention of the present study was to isolate and identify cultural conditions of potent fungal strain VJCH-19 with antagonistic activities isolated from mangrove ecosystems of Adavuladeevi, South coast, Andhra Pradesh, India. Methods: The soil samples were collected from the mangrove habitats and shade dried. The serial dilution plate technique was employed for the isolation of mangrove fungi by using Sabouraud dextrose agar and Czapek-Dox agar media. All the strains were screened for the production of antimicrobial compounds. The potential fungal strain was designated as VJCH-19. The identification of the strain was carried out based on cultural, micro-morphological and genomic characteristics. Results: The potent mangrove fungal strain VJCH-19 was identified as Simplicillium lanosoniveum. The secondary metabolites produced by the strain inhibited several bacteria and fungi. To check the nutrient adaptation, VJCH-19 was grown on different culture media and its morphological characters were observed. Conclusion: Among the twenty-five fungal strains, VJCH-19 was found to possess antimicrobial potential and the strain was identified as Simplicilium lanosoniveum...
Read More
Posted by admin on Jun 30, 2020 in |
Phymatosorus scolopendria (Burm. F.) Pic. Serm. belongs to the family Polypodiaceae which is commonly known as musk-fern or wart-fern. Medicinally, the experimental plant possesses insect repellent, anti-inflammatory and bronchodilator activity and hence used ethnomedicinally by tribal people, but was not explored for its bioactive compounds. Hence, the objective of this study was to determine the bioactive compounds present in the methanolic extract of Phymatosorus scolopendria by Gas Chromatography-Mass Spectrum. The methanolic extract of the experimental plant revealed the presence of four phytoconstituents, namely dodecanoic acid, 1,2,3-propanetriyl ester, myristic acid vinyl ester, 4-nitrophenyl laurate and hexadecanoic acid, 4-nitrophenyl ester with peak values 8.98, 53.29, 26.91 & 10.82. The predicted biological activities for the present compounds identified when referred in the literature shows antioxidant, antibacterial, antiviral, candidicidal, hypocholestrolemic, antiarthritic, hepatoprotective, mosquito repellent, antimicrobial, anticancer and cosmetics. The compounds were identified by comparing their retention time and peak area with that of literature and interpretation of mass spectra. The research could be continued to find out the therapeutic importance of all the...
Read More
Posted by admin on Jun 30, 2020 in |
A growing concern of microbial resistance and potential risks associated with existing synthetic preservatives have put industries and researchers under immense pressure to develop newer and safer alternatives based on moieties obtained from natural sources. The purpose of this study was to evaluate the preservative effect of the selected derivatives of naturally occurring phenylpropanoids. Aluminum Hydroxide Gel-USP was employed as a pharmaceutical formulation which has been challenged with Staphylococcus aureus MTCC 737, Escherichia coli MTCC 443, Pseudomonas aeruginosa MTCC 1688, Aspergillus niger MTCC 282 and Candida albicans MTCC 227 as per USP 2004. The selected ester and anilide derivatives demonstrated good antimicrobial potential against all the challenged microorganisms. The preservative efficacy results were comparable to that of standard preservatives, methyl, and propyl-parabens. The benzoin caffeate (CE48) derivative was found to be superior preservative amongst the other derivatives and as well as from both the standards mainly against S. aureus, A. niger and C. albicans. Thus, natural moiety based antimicrobial derivatives has the potential to be chosen as a preservative...
Read More
