Posted by admin on Dec 31, 2018 in |
A mathematical model optimizing bio-physico-chemical pre-treatment of steam exploded Piper nigrum, and Syzygium aromaticum lignocellulosic biomass waste was developed. The model was developed using, RSM, involving central composite face-centered design (CCD) with six parameters, three levels and 40 runs, at 95% confidence level. Six parameters optimized were quantity of each enzyme (a) Cellulase (ml); (b) Xylanase (ml) for enzymatic actions at 50 ºC; (c) Incubation time of 2-15 days for which substrate were dipped in various solvent systems; (d) Volume of solvent systems used; (e) Time of incubation at given steam pressure for steam explosion; and (f) Steam pressure 10-15 Psi. Cellulase and xylanase enzymes used were with activity (3.563 IU/mL), (33.32 IU/mL) respectively. ANOVA was applied for validation of the predicted model at 95% of confidence level. The Base 10 log transformation was selected from the comparative study, and the model predicted 13.79 µmoles/mL, the release of polyphenols at 2 days of incubation of substrate with 10 ml of the solvent system, under 15.0 psi steam explosion pressure...
Read More
Posted by admin on Dec 31, 2018 in |
In this study, we investigated anti-cancer and antimicrobial activity of Aegle marmelos leaf extracts and their chemical profile characterized by gas chromatography coupled mass spectrometry (GC-MS). A. marmelos leaves were extracted with acetone, methanol, ethanol, and chloroform. Presence of phenolic compounds was identified in these extracts by qualitative analysis. All the extracts were subjected for anti-bacterial activity against the different strains of bacteria (Staphylococcus aureus, Bacillus subtilis, Bacillus cereus, Bacillus ariyabattai, Bacillus megaterium, Pseudomonas putida, Klebsiella pneumonia, Serratia marcescens, and Escherichia coli). It is noteworthy that acetone extract elicited maximum growth inhibition on Serratia marcescens. Based on profound anti bacterial activity, acetone and methanol extract of A. marmelos were checked for cytotoxicity against MDA-MB-231, HEp-2 and vero cells. MDA-MB-231 cells were more sensitive to acetone extract of A. marmelos with an IC50 value of 79.62 µg/ml where as HEp-2 cells are more sensitive to methanol extract of A. marmelos with an IC50 value of 47.08 µg/ml. Vero cells withstand 24 h treatment of both extract, and it is evidenced...
Read More
Posted by admin on Dec 31, 2018 in |
The increasing failures of chemotherapeutics and antibiotic resistance exhibited by pathogenic microbes have led to the screening of several medicinal plants for their potential antimicrobial activity. Thus, the present endeavour deals with the exploration of antibacterial efficacy of Curcuma caesia (Roxb.) (Family Zingiberaceae) against bacterial cultures procured from IMTECH, Chandigarh as a screen for potential candidates for the development of natural antibiotics. The agar well diffusion assay was performed to screen antibacterial activity, and ZOI was recorded. The results were analyzed using one way ANOVA with DMRT. A broth macro-dilution assay was used to determine (MIC) and (MBC). Finally, the purified fraction was chemically characterized by spectral analysis. The antibacterial activity profile revealed that the methanolic root extract exhibited broad-spectrum antibacterial potentiality. The extract exhibiting the highest antibacterial activity was purified by column chromatography and further with HPLC, and TLC analyzed the band pattern. The bio-efficacy of the purified fraction was assessed against pathogenic bacteria, and the MIC and MBC were determined. The maximum zone of inhibition was recorded...
Read More
Posted by admin on Nov 30, 2018 in |
The whole plant extracts of N. crociformis and N. infundibulifolia was analyzed by priliminary phytochemical, total phenolics, flavonoid, carbohydrate and alkaloid content by standard methods. In-vitro antioxidant DPPH assay and Phosphomolybdenum assay developed by Braca et al., Prieto et al., respectively. In-vitro antibacterial (agar well diffusion) activity by gram negative Pseudomonas aeroginosa (ATCC 27853), gram positive (Streptococcus mutans MTCC 890 & Staphylococcus aureus ATCC 25923, Esteritia coli ATCC 25922) and antifungal activity by Aspergillus niger (ATCC 16404). The study shows that the petroleum ether, chloroform, ethanol and aqueous extract of the plants contains a good number of phytochemicals such as flavonoid, cardiac glycoside, saponin, alkaloid etc. as well as antioxidant with respect to the scavenging activity against free radicals DPPH which were roughly comparable to that of BHT. The plant extracts also exhibited potent antimicrobial activity against S. aureus, P. aeruginosa, S. mutans, E. coli and A. niger indicating its bactericidal and fungicidal properties. It may be concluded that the plant Nervilia crociformis is chemically more active than Nervilia...
Read More
Posted by admin on Nov 30, 2018 in |
Tecoma stans is a well-known traditional plant in India. The plant can be used in diabetes, digestive problems, and rheumatism and as antimicrobial. The aim of the study is to evaluate the anti-inflammatory activity of extracts of the stem bark of Tecoma stans (L.) by in-vitro and in-vivo methods. Ethyl acetate and alcoholic extract showed better anti-inflammatory activity by Human red blood cell membrane stabilization method. These extracts were also found to have significant inhibition to the carrageenan induced rat paw oedema. The gallic acid, a phenolic acid identified in the ethyl acetate fraction may contribute to the anti-inflammatory activity. The amount of gallic acid present in the ethyl acetate fraction of Tecoma stans (L.) was found to be 1.021 ng/mg extract, by HPTLC. The study concludes that the anti-inflammatory activity of stem bark of Tecoma stans may be due to the phenolics present in the extracts. Further studies are suggested to isolate and identify the more components responsible for the...
Read More
