Posted by admin on Jan 31, 2017 in |
The study involved both aqueous extraction and methanolic extraction of the powered leaves, which ensure all plant components are extracted for better results. Phytochemical analysis from the extracts was performed using the standard protocols. The antimicrobial susceptibility of the extracts was tested against E-coli, Pseudomonas and Klebsilla sp using agar disc diffusion method. Antioxidant property of the plant was evaluated using DPPH Method and Hydrogen Peroxide Scavenging activity method whereas antidiabetic property was evaluated using α-amylase inhibition assay. The plant extracts were used at varying concentrations to ensure which plant extract and concentration causes the most inhibition. Phytochemical analysis of the extract indicated the present of Saponin, phenol , phytosterol, steroid, terpenoid, flavanoid, carbohydrates and proteins while tannin, glycoside, plobatanin were absent. Anti-diabetic property of the plant was observed with a significant result of inhibiting a maximum of 83% of α-amylase activity (700µg/ml concentration of methanol extract) whereas antioxidant property with a maximum of 85% of (200µg/ml concentration of water extract) inhibition. Antimicrobial property of the extract shows effectiveness...
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Posted by admin on Jan 31, 2017 in |
The aim of this study was to analyze the chemical composition of dried clove buds volatile oil in Bangladesh by Gas Chromatography-Mass Spectrometry (GC-MS) technique and also show their medicinal properties according to the characteristics of the constituents present in that essential oil. In this experiment the clove buds essential oil was extracted by hydro distillation method. The major constituents obtained from the oil includes 3-Allyl-6-methoxyphenol i.e. m-Eugenol (69.44%), Eugenol acetate (10.79%), 4-hydroxy-4-mehtylpentan-2-one i.e. Tyranton (7.78%) Caryophyllene (6.80%), 1,4,7-Cycloundecatriene, 1,5,9,9-tetramethyl-,Z,Z,Z-and trace amounts (<1%) of other constituents. Eugenol is present in concentration of 69.43% which is the major volatile constituent of clove bud essential oil. It has wide range of medicinal value such as antiseptic and anaesthetic analgesic, antioxidant, anti-inflamatory and antimicrobial activities. Clove oil is used in dental care and it can relieve toothache temporarily. So, from these chemical constituents it is clear that clove buds essential oil of Bangladesh has medicinal properties because of the medicinal characteristics of these...
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Posted by admin on Jan 31, 2017 in |
Saponins are the foam producing secondary metabolites of plant. They have been evaluated for a number biological activities like anticancer, anti-inflammatory, hepatoprotective, antimicrobial, antitumor, blood cholesterol lowering properties etc. In the present study we have attempted to check the role of isolated saponins from Asparagus filicinus for its hypolipidemic activity. Saponin rich fraction of Asparagus filicinus was extracted by microwave assisted extraction technique. Phytochemical analysis and characterization was performed using FTIR technique comparing it to the standard spectra of saponins. Preliminary anti hyperlipidemic activity was evaluated using Triton WR 1339 model. This experiment shows significant reduction in total cholesterol, triglycerides, LDL and HDL level in comparison with Atorvastatin standard...
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Posted by admin on Jan 31, 2017 in |
Gas Chromatography-Mass Spectrometry (GC-MS) studies play an important role in isolation and identification of phytocompounds. Since, the chemical composition of the plants usually determines its biological activity, phytochemicals of possible pharmaceutical importance can be subjected to a detailed analysis. The present study is concerned with the GC-MS analysis of chloroform extract of the bark of Terminalia travancorensis, an evergreen tree, endemic to the Western Ghats. Reports on the benefits and or medicinal properties of T. travancorensis are rare or even absent. In this context, the present study was carried out to determine the chemical constituents of the bark by GC-MS analysis. Twenty two components were identified by GC-MS analysis in the chloroform extract. The major components detected were Friedelan-3-one (15.93%), gamma-sitosterol (10.48%), alpha -octadecene (8.54%), n-Tetracosanol-1 (8.21%), Phenol, 2,4-bis(1,1-dimethylethyl) (7.77%), 1-Nonadecene (7.49%), Heptacosanol (6.16%), 1-Heptadecene (5.25%) and stigmast-4-en-3-one (5.46 %). Friedelan-3-one, the major compound is a triterpenoid possessing antifeedant, anti-inflammatory, anticancer, hepatoprotective, antimicrobial and anticandidal activities. The second major compound, gamma-sitosterol plays a vital role in the structure and...
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Posted by admin on Jan 31, 2017 in |
In the present study 17 novel benzaldehyde thiosemicarbazone derivatives were synthesised using 2-methyl-3-thiosemicarbazide as the main reactant and different aromatic aldehydes. Reactions were monitored using thin layer chromatography technique and the newly synthesised derivatives were characterized by Elemental analysis, ATR and 1HNMR techniques. For antibacterial activity four bacterial strains were selected namely Bacillus subtilis (gram +ve), Staphylococcus aureus (gram +ve), Escherichia coli (gram –ve), and Pseudomonas aeruginosa (gram -ve).Candida albicans was used to ascertain the antifungal activity of compounds. Antimicrobial assay was performed using nutrient agar media for the bacterial strains and Sabouraud agar for the fungus, zone of inhibition of all the compounds which could inhibit visible growth after incubation were calculated. Four different dilutions of the test compounds were taken and compared against ciprofloxacin and fluconazole as the standard drugs. Antimicrobial assay was performed in duplicate. The test compounds were active against both gram negative as well as gram positive bacteria. Considerable anti-fungal was shown by the compounds. Antioxidant assay was performed using 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) or ABTS...
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