Posted by admin on Dec 31, 2016 in |
Acne is a cutaneous pleomorphic disorder of the pilosebaceous unit involving abnormalities in sebum production and is characterized by both inflammatory (papules, pustules and nodules) and noninflammatory (comedones, open and closed) lesions. A lot of factor viz; hormone, increased sebum production and diet causes Acne vulgaris but bacteria are the chief causal organism in which Propionibacterium acnes and Staphylococcus epidermidis are considered as the major skin bacteria that cause the formation of acne. The present study was conducted to evaluate the in vitro antimicrobial activities of Psidium guajava L. leaves essential oil against Propionibacterium acnes and Staphylococcus epidermidis. Extraction of essential oil was carried out using Clevenger’s Apparatus. Antibacterial activities of essential oil of leaves were investigated using broth micro dilution methods recommended by CLSI. The results showed that Guava leaves oils more effectively inhibit the growth of P.acnes (MIC: 0.321, IC50:0.309 mg/ml) as compared to S. epidermidis (MIC: 0.486, IC50:0.416 mg/ml). The readings were compared by standard drug...
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Posted by admin on Dec 31, 2016 in |
Simultaneous estimation method for Norfloxacin and Tinidazole in tablet dosage form had been developed by RP- HPLC. Both the drugs come under the category of antimicrobials and prescribed frequently in combination. The developed method is a gradient method which gives retention time of Norfloxacin and Tinidazole as 3.8min and 4.8min respectively. The separation was carried out on Supelco C18 (25cm × 4.6mm, 5µ) column using 0.05M phosphate buffer (pH 3 adjusted with ortho- phosphoric acid) and acetonitrile as a mobile phase. The flow rate was 0.8mL/min and wavelength used was 300nm. The method was validated as per ICH guideline and found to be linear between the range of 1- 6µg/mL for Norfloxacin and 1.5- 9µg/mL for Tinidazole. The developed method was found to be rapid, accurate and reproducible and therefore can be applied for simultaneous estimation of both the drugs as API or tablet dosage form...
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Posted by admin on Nov 30, 2016 in |
Blepharis molluginifolia (Acanthaceae), is a threatened medicinal herb. The methanolic flower extract of Blepharis molluginifolia was subjected to Silica Gel Column Chromatography for purification of bioactive compounds and structure elucidation of active compounds were studied. DPPH and antimicrobial activities of two isolated compounds were studied. From the analysed data, the compounds identified were 5,7-Dihydroxy-3-(4-methoxyphenyl)chromen-4-one (BiochaninA) C16H12O5 and 5,7-Dihydroxy-3-(4-hydroxyphenyl) chromen-4-one (Genistein) C15H10O5. These compounds are first time reported in this plant. Another fraction GF1 was subjected to GCMS analysis and obtained 12 compounds (aminoacid, gamma lactone, sugars and fatty acids).As GCMS profile contained majority of sugars antiglycation was done against arbutrin and found >70% inhibition. The present investigation revealed that the methanolic flower extract contained phytoestrogens like Biochanin A and Genistein, the rare aminoacid pyroglutamic acid, gamma lactones, sugars and fatty...
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Posted by admin on Oct 31, 2016 in |
The rhizosphere represents the thin layer of soil surrounding plant roots and the soil occupied by the roots, supports large active groups of microorganisms. The vast organic compounds (amino acids, sugars etc.) secreted by plant roots in the rhizosphere provide a food source for microorganisms increasing microbial biomass and their activity in the rhizosphere. Antibiotics are antimicrobial compounds produced by living microorganism as secondary metabolites. These compounds are used therapeutically and sometimes prophylactically in the control of infectious diseases. The isolation of antibiotics from microorganisms is relatively easy as compared to chemical synthesis of antimicrobial agents. The isolation of antibiotics from microorganisms improved the discovery of novel antibiotics that could act as better chemotherapeutic agents. With the increased population pressure, costs and side effects and the development of resistance of pathogens to drugs for infectious diseases, there is an urgent need to explore microbes for development of new antimicrobial metabolites. As microorganisms grow in unique and extreme habitats, they may have the capability to produce unique and unusual metabolites....
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Posted by admin on Sep 30, 2016 in |
The seeds of Vitis vinifera have been shown to have antioxidant, anticancer and antimicrobial properties due to the presence of rich bioactive phenolic components like resveratrol. The research deals with the synthesis of silver nanobioconjugates from the methanolic extract of grape seeds and the pure compound resveratrol. The presence of resveratrol was confirmed by HPLC analysis in methanolic extract of various parts of grape fruits such as skin, pulp and seeds of two varieties (pink and black). As higher amount of resveratrol was present in the seed extract of pink grapes, it was selected for the silver nanobioconjugate synthesis. Various conditions were applied to influence the rapid reduction of Ag+ to Agο, such as microwave heating, water bath heating, sunlight exposure and incubation at 37οC for nanobioconjugate synthesis. The reduction of silver ions was confirmed by color changes, adsorption spectroscopy (280-800nm) and percentage yield. Among the four different methods, sunlight exposure proved to be rapid in the synthesis and the yield of nanobioconjugates was also high. Therefore, this method...
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